Processing Options

Decalcification

We have experience with a wide variety of decalcification solutions designed for many different applications.

  • Our Faxitron UltraFocus allows daily assessment of decalcification if needed.
  • microCT is available

Paraffin

Our Excelsior Auto Tissue Processor has been optimized for high quality fixation and processing of bony tissues.

  • Vacuum lines enable faster infiltration, improving quality.
  • Hand processing protocols can be developed with users directly for cumbersome specimens.

Plastic

Get text from Doug/Julie about Plastics

Additional Processes Available

Fixation Strategies

  • We are equipped to assist in tissue and fixative preparation as needed.
  • Protocols are developed directly with users, and fixation procedures can be tailored to tissue-specific needs.
  • Custom fixative preparations and other requests can be easily accommodated.

Microtomy

  • Standard Microtomy is available for parafiin embedded specimens.
  • Plastic- and cryo-embedded specimens can be sectioned using our LLS Rowiak Tissue Surgeon 3.

Staining

  • We have auto staining capabilities for ### stains.
  • Special Stains including ***** are done by hand or require extra attention.
  • Immunohistochemical staining is done through our Roche Discovery Ultra

Digitization and Image Analysis

  • An Olympus VS200 scanner provides fast and high-quality digitization of any slides.
  • Bright field and fluorescent options (405 nm, 534 nm, 640 nm) are available.
  • Scoring algorithms for cartilage structural damage, chondrocyte counting, safranin O intensity, immunohistochemical intensity. All can be developed with users or applied here in OHSC.